Emil Khisamutdinov

Emil Khisamutdinov

Assistant Professor of Chemistry


Room:CP 305N

Postdoctoral Scholar, College of Pharmacy, University of Kentucky (2014)
Bowling Green State University Ph.D. (2012)
National University of Uzbekistan M.S. (2007)
National University of Uzbekistan B.S. (2005)

Related Link:
Emil Research Group

Research summary:

Our laboratory is focused on RNA nanotechnology and involves the design, construction, and application of RNA based nanoparticles. RNA nanotechnology is a vigorous, and rapidly emerging with strong potential for application in diverse fields from nanoelectronics to nanomedicine. RNA molecules not only encode genetic information, but they actively participate in various intracellular functions including gene expression regulation through sophisticated mechanisms; thereby expanding its traditional role as a genetic messenger and revealing it as a functionally versatile molecule. Small interfering and microRNAs (siRNAs and miRNAs, respectively), ribozymes, rib switches, ribosomal RNA, transport RNA (tRNA) are only a few examples of non-coding RNA (ncRNA) elements that play diverse roles in mediating gene expression. To achieve their functions, RNAs fold into a complex three dimensional (3D) architectures. Inspired by these natural 3D RNA elements, the development of artificial or reengineered RNA nanoparticles that will efficiently function in variety of intracellular processes as “smart” nanodevises is our main research interest. 


Our research projects are highly interdisciplinary and combine chemistry, biology, physics and material science. Our ultimate goal is the development of new strategies for controlled self-assembly of functional RNA nanoparticles with implications in areas as diverse as nanoelectronics, biosencing, and nanomedicine. Our projects provide students with extensive training in RNA 3D design and with various biochemical techniques including DNA/RNA labeling, PCR, RNA synthesis (in vitro transcription), gel electrophoresis, protein over expression and isolation, Fluorescent and UV- Vis spectroscopy, UV-melting, Atomic Force Microscopy (AFM), Transmission Electron Microscopy (TEM).

Selected publication:

  1. Seth G. Abels and Emil F. Khisamutdinov. Nucleic Acid Computing and its Potential to Transform Silicon-Based Technology. DNA and RNA Nanotechnology. 2015. Vol:2 (1), pp 13-22. (DOI: 10.1515/rnan-2015-0003)
  2. Emil F. Khisamutdinov, Alexey E. Shamaev, Philip A. Boda, Kanikey Karabaeva, Andrey Mereshenko, Alexander N. Tarnovsky, Neocles B. Leontis, R. Marshall Wilson. A Pyrene Dihydrodioxin with Pyridinium “Arms”: A Photochemically Activatable DNA Cleaving Agent with Unusual Duplex Stabilizing Properties. Journal of Photochemistry and Photobiology A: Chemistry, 2015. Vol: 307-308, pp 131-146. (doi:10.1016/j.jphotochem.2015.03.017)
  3. Emil F. Khisamutdinov, My Nguyen Hoan Bui, Daniel Jasinski, Zhengyi Zhao, Zheng Cui, Peixuan Guo. Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60° to 90° or 108°. In RNA Scaffolds: Methods and Protocols, Methods in Molecular Biology edited by L. Ponchon. Springer, 2015. Vol: 1316; pp181-193. (doi: 10.1007/978-1-4939-2730-2_15)
  4. Emil F. Khisamutdinov, Hui Li, Daniel L. Jasinski, Jiao Chen, Jian Fu, and Peixuan Guo. Enhancing Immunomodulation on Innate Immunity by Shape Transition Among RNA Triangle, Square, and Pentagon Nanovehicles. Nucleic Acids Research, 2014, 42(15):9996-10004. (doi: 10.1093/nar/gku516)
  5. Emil F. Khisamutdinov, Daniel L. Jasinski, and Peixuan Guo. RNA as a Boiling-Resistant Anionic Polymer Material To Build Robust Structures with Defined Shape and Stoichiometry. ACS Nano, 2014, 8 (5), pp 4771–4781. (doi: 10.1021/nn5006254)  
  6. Daniel Binzel, Emil F. Khisamutdinov, and Peixuan Guo. Entropy-driven one-step formation of Phi29 pRNA 3WJ from three RNA fragments. Biochemistry, 2014, 53 (14), pp 2221–2231. (doi: 10.1021/bi4017022)
  7. Daniel L. Jasinski, Emil F. Khisamutdinov, and Peixuan Guo. Physicochemically Tunable Polyfunctionalized RNA Square Architecture with Fluorogenic and Ribozymatic Properties Properties. ACS Nano, 2014, 8 (8), pp 7620–7629. (DOI: 10.1021/nn502160s) 
  8. Zhao Zhengyi, Emil F. Khisamutdinov, Chad Schwartz, and Peixuan Guo. Mechanism of One-Way Traffic of Hexameric Phi29 DNA Packaging Motor with Four Electropositive Relaying Layers Facilitating Antiparallel Revolution. ACS Nano. 2013, 28;7(5), pp 4082-4092. (doi: 10.1021/nn4002775) 
  9. Meikang Qiu, Emil F. Khisamutdinov, Zhengyi Zhao, Cheryl Pan, Jeong-Woo Choi, Neocles B. Leontis, Peixuan Guo. RNA nanotechnology for computer design and in vivo computation. Phil. Trans. R. Soc. A., 2013. 371: 1471-2962. (doi: 10.1098/rsta.2012.0310)
  10. Neocles B. Leontis and Emil F. Khisamutdinov. RNA Nanotechnology: Learning from Biologically active RNA Nano-machines. In RNA Nanotechnology and Therapeutics edited by P. Guo and F. Haque. CRC press, Taylor and Francis Group, 2013. Chapter #4; 73-108
  11. Dan Shu, Emil F. Khisamutdinov, Le Zhang, and Peixuan Guo. Programmable folding of fusion RNA in vivo and in vitro driven by pRNA 3WJ motif of phi29 DNA packaging motor. Nucleic Acid Research, 2013. (doi: 10.1093/nar/gkt885)