James Olesen

James Olesen

Assistant Professor of Biology

Phone:765-285-3510

Room:CL 229D


Related Link
Personal Web site

Educational Background

Bowling Green State University, Ph.D. ,1994
The Ohio State University, M.S. ,1987
Bowling Green State University, B.S. ,1983

TAL-1 transcription factor

Research Interests

My general interests are in molecular and cancer biology.

Specific Goal: Understanding how ectopic expression of the transcription factor, TAL-1 (see Fig. 1), contributes to leukemogenesis. Through ill-defined mechanisms, TAL-1 seems to play a significant role in the progression of T-cell acute lymphoblastic leukemia. It is thought that important cellular processes such as cell cycle control, cell signaling and apoptosis (see Fig. 2) are influenced by this mis-expressed protein. Through investigating


Image from Cell Signaling Technology website

potential targets of TAL-1, it is hoped that a better picture of how TAL-1 influences apoptotic resistance and cell survival will be realized. In the end, this type of research will hopefully guide the future development of better, more targeted therapies to treat T-ALL at the molecular level.

In our research, we:

  • Assess protein expression in isolated cellular extracts via Western blotting.
  • Characterize intracellular protein expression levels though the use of flow cytometry.
  • Utilize immunofluorescence and confocal microscopy to investigate the localization of various proteins within cells (see Fig. 3).
  • Study the apoptotic response in the malignant Jurkat cell line as measured by caspase activity and TUNEL assays.

Recent Publications:

Bombuwala, K., Kinstle, t., Popik, V., Uppal, S., Olesen, J.B., Vina, J. and Heckman, C.A. (2006). Colchitaxel, A Coupled Compound Made from Microtubule Inhibitors Colchcine and Paclitaxel. Beilstein Journal of Organic Chemistry 2:13-22.

Olesen, J.B. and Gough, E. (2006). Optimization of Transfection Conditions for the Human HL-60 Promyelocytic Leukemic T-Cell Line Using FuGene HD Transfection Reagent. Biochemica, 3:24-25.

Lucas, B. and Olesen, J.B. Ectopic expression of TAL-1 increases resistance to TNFα-induced apoptosis in Jurkat cells via changes in the NF-κB signaling pathway. In preparation.